Experiment summary for ENCSR529PIJ

doi:10.17989/ENCSR529PIJ

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
F49E8.2
Biosample summary
Caenorhabditis elegans strain OP383 (unc-119(tm4063) III; wgIs383) whole organism hermaphrodite young adult 48 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans oef-1
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing F49E8.2-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
E-Gel
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:F49E8.2-OP383-yAd
Date submitted
July 30, 2015
Date released
November 22, 2017
Submitter comment
This experiment was completed during modENCODE and has lower read depth than the current modERN standard.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
31Caenorhabditis elegans strain OP383 (unc-119(tm4063) III; wgIs383) hermaphrodite young adult 48 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans oef-1ENCBS957WQGENCGM637QPJENCAB435FBQENCLB352LNE
21Caenorhabditis elegans strain OP383 (unc-119(tm4063) III; wgIs383) hermaphrodite young adult 48 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans oef-1ENCBS156BARENCGM637QPJENCAB435FBQENCLB521IRZ
11Caenorhabditis elegans strain OP383 (unc-119(tm4063) III; wgIs383) hermaphrodite young adult 48 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans oef-1ENCBS261HTKENCGM637QPJENCAB435FBQENCLB912PIE