Experiment summary for ENCSR529PIJ
doi:10.17989/ENCSR529PIJ
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- F49E8.2
- Biosample summary
- Caenorhabditis elegans strain OP383 (unc-119(tm4063) III; wgIs383) whole organism hermaphrodite young adult 48 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans oef-1
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing F49E8.2-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- E-Gel
- Platform
- Illumina Genome Analyzer IIx
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:F49E8.2-OP383-yAd
- Date submitted
- July 30, 2015
- Date released
- November 22, 2017
- Submitter comment
- This experiment was completed during modENCODE and has lower read depth than the current modERN standard.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
3 | 1 | Caenorhabditis elegans strain OP383 (unc-119(tm4063) III; wgIs383) hermaphrodite young adult 48 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans oef-1 | ENCBS957WQG | ENCGM637QPJ | ENCAB435FBQ | ENCLB352LNE |
2 | 1 | Caenorhabditis elegans strain OP383 (unc-119(tm4063) III; wgIs383) hermaphrodite young adult 48 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans oef-1 | ENCBS156BAR | ENCGM637QPJ | ENCAB435FBQ | ENCLB521IRZ |
1 | 1 | Caenorhabditis elegans strain OP383 (unc-119(tm4063) III; wgIs383) hermaphrodite young adult 48 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans oef-1 | ENCBS261HTK | ENCGM637QPJ | ENCAB435FBQ | ENCLB912PIE |