Experiment summary for ENCSR528ASX

doi:10.17989/ENCSR528ASX

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
GRWD1
Biosample summary
Homo sapiens K562 expressing RNAi targeting H. sapiens GRWD1
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on K562 cells treated with an shRNA knockdown against GRWD1. (GRWD1_BGKLV21)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
8.7 [1-1]9.6 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:GRWD1_BGKLV21
Date submitted
August 14, 2015
Date released
August 18, 2015
Gene Silencing Series
ENCSR424NXX 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line expressing RNAi targeting H. sapiens GRWD1ENCBS654PMTENCGM676OUHENCLB311FRB
21Homo sapiens K562 cell line expressing RNAi targeting H. sapiens GRWD1ENCBS861PFYENCGM676OUHENCLB998LYB