1. Experiments
  2. ChIP-seq
  3. Caenorhabditis elegans
  4. whole organism

Experiment summary for ENCSR518VJU

doi:10.17989/ENCSR518VJU

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
mel-28
Biosample summary
Caenorhabditis elegans strain OP646 (unc-119(tm4063) III; wgIs646) whole organism young adult 64 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans mel-28
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing MEL-28-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:MEL-28-OP646-yAd
Date submitted
October 4, 2016
Date released
October 10, 2016
Submitter comment
ENCFF982AXZ, ENCFF095YDR, ENCFF934THB, ENCFF693TPA were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Caenorhabditis elegans strain OP646 (unc-119(tm4063) III; wgIs646) mixed sex young adult 64 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans mel-28ENCBS248YNTENCGM749TPTENCAB728YTOENCLB738BRX
11Caenorhabditis elegans strain OP646 (unc-119(tm4063) III; wgIs646) mixed sex young adult 64 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans mel-28ENCBS561UNGENCGM749TPTENCAB728YTOENCLB524YFC

Files

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Replicates

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