Experiment summary for ENCSR504LBE

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
JUN
Biosample summary
Homo sapiens A549 treated with 100 nM dexamethasone for 5 hours
Biosample Type
cell line
Replication type
isogenic
Description
cJun ChIP-seq on A549 cell line treated with 100 nM dexamethasone for 5 hours.
Treatments
100 nM dexamethasone (CHEBI:41879) for 5 hours
Nucleic acid type
DNA
Library starting quantity
7ng
Size range
>200
Fragmentation methods
sonication (Bioruptor Twin)
Size selection method
dual SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

Genomics of Gene Regulation Project
Lab
Tim Reddy, Duke
Award
U01HG007900 (Tim Reddy, Duke)
Project
GGR
External resources
Aliases
tim-reddy:cJun.t5
Date submitted
December 15, 2016
Date released
December 20, 2016
Related datasets
ENCSR588JLN 
ChIP-seq experiments using a 100 nM dexamethasone (CHEBI:41879) treatment and targeting JUN.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Antibody
Library
11A549 cell line treated with 100 nM dexamethasone for 5 hoursENCBS168IFTENCAB085XKXENCLB287CBS
21A549 cell line treated with 100 nM dexamethasone for 5 hoursENCBS471WAIENCAB085XKXENCLB718QTY
31A549 cell line treated with 100 nM dexamethasone for 5 hoursENCBS951GWLENCAB085XKXENCLB988JSG