Experiment summary for ENCSR480OHP
doi:10.17989/ENCSR480OHP
Summary
- Status
- released
- Assay
- ChIP-seq (Histone ChIP-seq)
- Target
- H3K27ac
- Biosample summary
- Homo sapiens A549 treated with 100 nM dexamethasone (agonist) for 25 minutes
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- H3K27ac ChIP-seq on A549 cell line treated with 100 nM dexamethasone for 25 mins.
- Treatments
- 100 nM dexamethasone (CHEBI:41879) for 25 minutes
- Nucleic acid type
- DNA
- Library starting quantity
- 7ng
- Size range
- >200
- Fragmentation methods
- sonication (Bioruptor Twin)
- Size selection method
- dual SPRI
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
Genomics of Gene Regulation Project
- Lab
- Tim Reddy, Duke
- Award
- U01HG007900 (Tim Reddy, Duke)
- Project
- GGR
- External resources
- Aliases
- tim-reddy:H3K27ac.t25m
- Date submitted
- October 13, 2017
- Date released
- October 19, 2017
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Antibody | Library |
|---|---|---|---|---|---|
| 3 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 25 minutes | ENCBS334AKK | ENCAB037IXK | ENCLB728GFT |
| 1 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 25 minutes | ENCBS978CEM | ENCAB037IXK | ENCLB352VET |
| 2 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 25 minutes | ENCBS128BGV | ENCAB037IXK | ENCLB199OME |