Experiment summary for ENCSR450HPD
doi:10.17989/ENCSR450HPD
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- bsh
- Biosample summary
- Drosophila melanogaster strain bsh-GFP whole organism embryo (0-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster bsh
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing bsh-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:bsh-GFP
- Date submitted
- December 21, 2016
- Date released
- January 10, 2017
- Submitter comment
- ENCFF570RYE, ENCFF219FVC, ENCFF231PBO, ENCFF822QNS were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
1 | 1 | Drosophila melanogaster strain bsh-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster bsh | ENCBS349CWV | ENCGM470HST | ENCAB728YTO | ENCLB862EKY |
2 | 1 | Drosophila melanogaster strain bsh-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster bsh | ENCBS226CLG | ENCGM470HST | ENCAB728YTO | ENCLB730QOF |