Experiment summary for ENCSR450HPD

doi:10.17989/ENCSR450HPD

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
bsh
Biosample summary
Drosophila melanogaster strain bsh-GFP whole organism embryo (0-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster bsh
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing bsh-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:bsh-GFP
Date submitted
December 21, 2016
Date released
January 10, 2017
Submitter comment
ENCFF570RYE, ENCFF219FVC, ENCFF231PBO, ENCFF822QNS were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Drosophila melanogaster strain bsh-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster bshENCBS349CWVENCGM470HSTENCAB728YTOENCLB862EKY
21Drosophila melanogaster strain bsh-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster bshENCBS226CLGENCGM470HSTENCAB728YTOENCLB730QOF