Experiment summary for ENCSR423XRG
doi:10.17989/ENCSR423XRG
Summary
- Status
- released
- Assay
- single-cell RNA sequencing assay (scRNA-seq)
- Control type
- control
- Biosample summary
- Homo sapiens pyramidal neuron male adult (20 years)
- Biosample Type
- primary cell
- Replication type
- isogenic
- Description
- RNA-seq on 30 cell pools of excised pyramidal cells from a 20 year old human male
- Nucleic acid type
- RNA
- Library starting quantity
- 30cells
- Size range
- >200
- Lysis method
- 72 degrees for 3 minutes in the presence of Triton
- Fragmentation methods
- chemical (Nextera tagmentation)
- Size selection method
- SPRI beads
- Strand specificity
- unstranded
- Spike-ins datasets
- ENCSR013YHQ
- Platform
- Illumina HiSeq 2000
Attribution
ENCODE3 project
- Lab
- Barbara Wold, Caltech
- Award
- U54HG006998 (Richard Myers, HAIB)
- Project
- ENCODE
- External resources
- Aliases
- barbara-wold:Hs_UMB4727_20_M_CN_midFI_pyramids_30pool
- Date submitted
- May 13, 2014
- Date released
- July 7, 2014
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Homo sapiens male adult (20 years) pyramidal neuron primary cell | ENCBS590LDH | ENCLB164LQL |
| 2 | 1 | Homo sapiens male adult (20 years) pyramidal neuron primary cell | ENCBS651ADZ | ENCLB621DLJ |