Experiment summary for ENCSR350EFV
doi:10.17989/ENCSR350EFV
Summary
- Status
- released
- Assay
- ChIP-seq (Histone ChIP-seq)
- Target
- H3K27ac
- Biosample summary
- Homo sapiens A549 treated with 100 nM dexamethasone (agonist) for 3 hours
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- H3K27ac ChIP-seq on A549 cell line treated with 100 nM dexamethasone for 3 hours.
- Treatments
- 100 nM dexamethasone (CHEBI:41879) for 3 hours
- Nucleic acid type
- DNA
- Library starting quantity
- 7ng
- Size range
- >200
- Fragmentation methods
- sonication (Bioruptor Twin)
- Size selection method
- dual SPRI
- Platform
- Illumina HiSeq 2500, Illumina HiSeq 2000
- Controls
Attribution
Genomics of Gene Regulation Project
- Lab
- Tim Reddy, Duke
- Award
- U01HG007900 (Tim Reddy, Duke)
- Project
- GGR
- External resources
- Aliases
- tim-reddy:H3K27ac.t3
- Date submitted
- May 30, 2016
- Date released
- July 21, 2016
- Treatment Time Series
- ENCSR375BQN
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Antibody | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 3 hours | ENCBS714CHO | ENCAB037IXK | ENCLB507KDK |
| 2 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 3 hours | ENCBS107VVO | ENCAB037IXK | ENCLB700CQP |
| 3 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 3 hours | ENCBS492GFZ | ENCAB037IXK | ENCLB471PWH |