Experiment summary for ENCSR320IEK
doi:10.17989/ENCSR320IEK
Supersedes ENCSR137HKS
Summary
- Status
- released
- Assay
- CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
- Target
- PRPF8
- Biosample summary
- Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens PRPF8
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RNA-seq on K562 cells treated with a CRISPR gRNA against PRPF8. (PRPF8-BGKcLV08)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.7 [1-1]9.9 [2-1]
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR156CIL
- Platform
- Illumina NovaSeq 6000
- Controls
Attribution
ENCODE4 project
- Lab
- Brenton Graveley, UConn
- Award
- U41HG009889 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:PRPF8-BGKcLV08
- Date submitted
- May 23, 2019
- Date released
- November 3, 2020
- Gene Silencing Series
- ENCSR217BVW
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens PRPF8 | ENCBS820MRK | ENCGM472MGY | ENCLB066CFQ |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens PRPF8 | ENCBS235VRF | ENCGM472MGY | ENCLB464IQH |