Experiment summary for ENCSR307BCA

Summary

Status
released
Assay
RNA-seq (polyA RNA-seq)
Biosample summary
Mus musculus C57BL/6 midbrain embryo (11.5 days)
Biosample Type
tissue
Replication type
isogenic
Description
PolyA RNA-Seq from oligo-dT primed Total RNA on embryonic 11.5 day (e11.5) mouse midbrain
Nucleic acid type
polyadenylated mRNA
RNA integrity number
10
Library starting quantity
10ng
Size range
>200
Lysis method
Ambion mirVana
Extraction method
Ambion mirVana
Fragmentation method
chemical (Nextera tagmentation)
Size selection method
SPRI beads
Strand specificity
Non-strand-specific
Spike-ins datasets
ENCSR884LPM

Attribution

ENCODE3 project
Lab
Barbara Wold, Caltech
Award
U54HG006998 (Richard Myers, HAIB)
Project
ENCODE
External resources
Date submitted
October 29, 2014
Date released
November 20, 2014
Related datasets
ENCSR505AHT 
RNA-seq on mouse midbrain (embryonic 11.5 day - postnatal).
, ENCSR843IAS 
Reference epigenome for mouse midbrain (embryonic 11.5 day).
Tags
Search for all Experiment with internal tag ENCYCLOPEDIAv4

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleLibrary
21strain B6NCrl mixed sex embryo (11.5 days) midbrain tissueENCBS802FTIENCLB426JKF
11strain B6NCrl mixed sex embryo (11.5 days) midbrain tissueENCBS228OBSENCLB659UFY