Experiment summary for ENCSR295PZT

Summary

Status
released
Assay
RNA-seq (total RNA-seq)
Biosample summary
Mus musculus C57BL/6 bone marrow-derived macrophage nuclear fraction
Biosample Type
primary cell
Replication type
isogenic
Description
Ribo depletion RNA-seq on nucleoplasmic fraction RNA which is part of BMDM cell pre-treated with 100 ng/ml Lipid A 12 hours, washed out for 12 hours, and stimulated with 100 ng/ml Lipid A for 40 minute.
Nucleic acid type
RNA
Depleted in
rRNA
Library starting quantity
500ng [2-1]1µg [1-1]
Size range
250-400
Extraction method
see document
Fragmentation method
see document
Size selection method
SPRI beads
Strand specificity
Non-strand-specific

Attribution

Genomics of Gene Regulation Project
Lab
Alexander Hoffmann, UCLA
Award
U01HG007912 (Alexander Hoffmann, UCLA)
Project
GGR
Aliases
alexander-hoffmann:Nuc.LPA.040.exp
Date submitted
January 20, 2017
Date released
November 20, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleLibrary
21strain C57BL/6J bone marrow-derived macrophage primary cell nuclear fractionENCBS933JVUENCLB962TZG
11strain C57BL/6J bone marrow-derived macrophage primary cell nuclear fractionENCBS361DEKENCLB578HVF