Experiment summary for ENCSR264TUE
doi:10.17989/ENCSR264TUE
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Control type
- non-specific target
- Biosample summary
- Homo sapiens HepG2 expressing RNAi
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Control shRNA against no target in HepG2 cells followed by RNA-seq. (NT_BGHLV35)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 10
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR358OMW
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:NT_BGHLV35
- Date submitted
- September 25, 2016
- Date released
- September 29, 2016
- Gene Silencing Series
- ENCSR019AYN , ENCSR889URB , ENCSR287SRR , ENCSR326YSW , ENCSR968OBA , ENCSR627GST , ENCSR144FRZ , ENCSR825EXD , ENCSR100DSP , ENCSR738DNW , ENCSR370RIN , ENCSR109KPD
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens HepG2 cell line expressing RNAi | ENCBS542QTQ | ENCGM266AWP | ENCLB351LSE |
| 1 | 1 | Homo sapiens HepG2 cell line expressing RNAi | ENCBS599TQI | ENCGM266AWP | ENCLB718NAR |