Experiment summary for ENCSR222SMI

doi:10.17989/ENCSR222SMI

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
RBM27
Biosample summary
Homo sapiens HepG2 expressing RNAi targeting H. sapiens RBM27
Biosample Type
cell line
Replication type
isogenic
Description
shRNA knockdown against RBM27 in HepG2 cells followed by RNA-seq. (RBM27_BGHLV18)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.4
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:RBM27_BGHLV18
Date submitted
December 11, 2014
Date released
December 17, 2014
Gene Silencing Series
ENCSR603CKC 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens RBM27ENCBS851ZNEENCGM175NOHENCLB679PHQ
11Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens RBM27ENCBS646RDVENCGM175NOHENCLB195HJB