Experiment summary for ENCSR220DTX
doi:10.17989/ENCSR220DTX
Summary
- Status
- released
- Assay
- CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
- Control type
- non-specific target
- Biosample summary
- Homo sapiens K562 genetically modified (deletion) using CRISPR
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Control CRISPR gRNA against no target in K562 cells followed by RNA-seq. (NT-BGKcLV28)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.5
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR156CIL
- Platform
- Illumina NovaSeq 6000
Attribution
ENCODE4 project
- Lab
- Brenton Graveley, UConn
- Award
- U41HG009889 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:NT-BGKcLV28
- Date released
- November 19, 2021
- Gene Silencing Series
- ENCSR523OQC , ENCSR831INO , ENCSR292MQU , ENCSR608EQY , ENCSR249YGF , ENCSR665JUV , ENCSR682PUE , ENCSR661PBE , ENCSR285JMR , ENCSR218HPV , ENCSR221MPE , ENCSR837ZEJ , ENCSR230BMV , ENCSR946GVC , ENCSR037JLU , ENCSR582NNR , ENCSR053TLP , ENCSR169WSA , ENCSR082GWT , ENCSR909SOX , ENCSR123TYO , ENCSR646GHV , ENCSR986FVB , ENCSR433VPP , ENCSR169PVW , ENCSR545FZJ , ENCSR627QEI
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line genetically modified (deletion) using CRISPR | ENCBS735BVV | ENCGM474NKS | ENCLB253YLV |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (deletion) using CRISPR | ENCBS225NCG | ENCGM474NKS | ENCLB911CEZ |