Experiment summary for ENCSR200GOV
doi:10.17989/ENCSR200GOV
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- brk
- Biosample summary
- Drosophila melanogaster strain brk-GFP whole organism wandering third instar larva genetically modified (insertion) using site-specific recombination targeting D. melanogaster brk
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing brk-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:brk-GFP
- Date submitted
- July 23, 2015
- Date released
- July 6, 2016
- Submitter comment
- ENCFF280JTV, ENCFF886XYW, ENCFF768PLR, ENCFF851VLE were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 3 | 1 | Drosophila melanogaster strain brk-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster brk | ENCBS694XCB | ENCGM140KKL | ENCAB728YTO | ENCLB073MAU |
| 4 | 1 | Drosophila melanogaster strain brk-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster brk | ENCBS988RCT | ENCGM140KKL | ENCAB728YTO | ENCLB063JZK |