Experiment summary for ENCSR181RLB

doi:10.17989/ENCSR181RLB

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
EEF2
Biosample summary
Homo sapiens K562 expressing RNAi targeting H. sapiens EEF2
Biosample Type
cell line
Replication type
isogenic
Description
shRNA knockdown against EEF2 in K562 cells followed by RNA-seq. (EEF2_BGKLV19)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.9 [1-1]10 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:EEF2_BGKLV19
Date submitted
December 11, 2014
Date released
December 17, 2014
Gene Silencing Series
ENCSR396EPO 
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv1Search for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line expressing RNAi targeting H. sapiens EEF2ENCBS198UTWENCGM911QNOENCLB499ERT
21Homo sapiens K562 cell line expressing RNAi targeting H. sapiens EEF2ENCBS715XGHENCGM911QNOENCLB825VKF