Experiment summary for ENCSR168NHD

doi:10.17989/ENCSR168NHD

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
CrebA
Biosample summary
Drosophila melanogaster strain CrebA-MiMIC-GFP whole organism wandering third instar larva genetically modified (insertion) using site-specific recombination targeting D. melanogaster CrebA
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing CrebA-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:CrebA-MIMIC-GFP
Date submitted
July 23, 2015
Date released
July 6, 2016
Submitter comment
ENCFF509AUY and ENCFF790JHL were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
41Drosophila melanogaster strain CrebA-MiMIC-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster CrebAENCBS088KOAENCGM220CXQENCAB728YTOENCLB088CAX
21Drosophila melanogaster strain CrebA-MiMIC-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster CrebAENCBS961OFOENCGM220CXQENCAB728YTOENCLB372MYC