Experiment summary for ENCSR167JPY

doi:10.17989/ENCSR167JPY

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
SRP68
Biosample summary
Homo sapiens HepG2 expressing RNAi targeting H. sapiens SRP68
Biosample Type
cell line
Replication type
isogenic
Description
shRNA knockdown against SRP68 in HepG2 cells followed by RNA-seq. (SRP68_BGHLV17)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.6
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:SRP68_BGHLV17
Date submitted
February 21, 2015
Date released
December 17, 2014
Gene Silencing Series
ENCSR579VHH 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens SRP68ENCBS885ORMENCGM005GTRENCLB601WEK
21Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens SRP68ENCBS061GZIENCGM005GTRENCLB935AWZ