Experiment summary for ENCSR149ZRX

doi:10.17989/ENCSR149ZRX

Summary

Status
released
Assay
PRO-cap
Biosample summary
Homo sapiens K562
Biosample Type
cell line
Replication type
isogenic
Description
CoPRO experiment. A run-on assay was performed on captured nascent RNA with cap state selection. In this experiment, both capped and uncapped nascent RNA was selected and PCR amplification was performed before sequencing.
Nucleic acid type
RNA
Average fragment size
44 [1-1]45 [2-1]
Size selection method
Streptavidin magnetic beads
Library construction method
capped RNA enrichment, uncapped RNA enrichment
Strand specificity
Strand-specific (reverse)

Attribution

ENCODE4 project
Lab
John Lis, Cornell
Award
UM1HG009393 (John Lis, Cornell)
Project
ENCODE
Aliases
john-lis:copro-experiment-CappedAndUncapped-Amplified
Date submitted
November 19, 2021
Date released
December 14, 2021

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Homo sapiens K562 cell lineENCBS526RISENCLB875MQQ
21Homo sapiens K562 cell lineENCBS396XJPENCLB888WSM