Experiment summary for ENCSR149ZRX
doi:10.17989/ENCSR149ZRX
Summary
- Status
- released
- Assay
- PRO-cap
- Biosample summary
- Homo sapiens K562
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- CoPRO experiment. A run-on assay was performed on captured nascent RNA with cap state selection. In this experiment, both capped and uncapped nascent RNA was selected and PCR amplification was performed before sequencing.
- Nucleic acid type
- RNA
- Average fragment size
- 44 [1-1]45 [2-1]
- Size selection method
- Streptavidin magnetic beads
- Library construction method
- capped RNA enrichment, uncapped RNA enrichment
- Strand specificity
- Strand-specific (reverse)
- Platform
- Illumina NextSeq 500
Attribution
ENCODE4 project
- Lab
- John Lis, Cornell
- Award
- UM1HG009393 (John Lis, Cornell)
- Project
- ENCODE
- External resources
- Aliases
- john-lis:copro-experiment-CappedAndUncapped-Amplified
- Date submitted
- November 19, 2021
- Date released
- December 14, 2021
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line | ENCBS526RIS | ENCLB875MQQ |
| 2 | 1 | Homo sapiens K562 cell line | ENCBS396XJP | ENCLB888WSM |