Experiment summary for ENCSR118KUN

doi:10.17989/ENCSR118KUN

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
DKC1
Biosample summary
Homo sapiens HepG2 expressing RNAi targeting H. sapiens DKC1
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on HepG2 cells treated with an shRNA knockdown against DKC1. (DKC1_BGHLV31)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.9 [1-1]10 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:DKC1_BGHLV31
Date submitted
March 7, 2016
Date released
March 16, 2016
Gene Silencing Series
ENCSR502RXV 
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv1Search for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens DKC1ENCBS152RQCENCGM563OYZENCLB498RQT
21Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens DKC1ENCBS813FSCENCGM563OYZENCLB826EYR