Experiment summary for ENCSR117TRB

doi:10.17989/ENCSR117TRB

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
lin-54
Biosample summary
Caenorhabditis elegans strain OP743 (unc-119(tm4063); wgIs743 [lin-54::TY1::EGFP::3xFLAG(Fosmid: lin-54) + unc-119(+)]) whole organism young adult (65 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans lin-54
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing lin-54-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:LIN-54_OP743_yAd
Date submitted
February 26, 2018
Date released
March 1, 2018

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Caenorhabditis elegans strain OP743 (unc-119(tm4063); wgIs743 [lin-54::TY1::EGFP::3xFLAG(Fosmid: lin-54) + unc-119(+)]) mixed sex young adult (65 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans lin-54ENCBS628IZZENCGM568NMRENCAB728YTOENCLB122OYU
11Caenorhabditis elegans strain OP743 (unc-119(tm4063); wgIs743 [lin-54::TY1::EGFP::3xFLAG(Fosmid: lin-54) + unc-119(+)]) mixed sex young adult (65 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans lin-54ENCBS915NTCENCGM568NMRENCAB728YTOENCLB911ZRS