Experiment summary for ENCSR107BQR
doi:10.17989/ENCSR107BQR
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- nhr-85
- Biosample summary
- Caenorhabditis elegans strain OP539 (unc-119(tm4063) III; wgIs539) whole organism hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans nhr-85
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing NHR-85-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:NHR-85-OP539-L1
- Date submitted
- July 29, 2015
- Date released
- May 24, 2016
- Submitter comment
- ENCFF627TGM, ENCFF824ILX, ENCFF018KHA, ENCFF152NGD were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | Caenorhabditis elegans strain OP539 (unc-119(tm4063) III; wgIs539) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans nhr-85 | ENCBS989DRZ | ENCGM287GCK | ENCAB728YTO | ENCLB441ODB |
| 1 | 1 | Caenorhabditis elegans strain OP539 (unc-119(tm4063) III; wgIs539) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans nhr-85 | ENCBS219LUD | ENCGM287GCK | ENCAB728YTO | ENCLB169AGL |