Experiment summary for ENCSR094KBY

doi:10.17989/ENCSR094KBY

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
SRSF1
Biosample summary
Homo sapiens HepG2 expressing RNAi targeting H. sapiens SRSF1
Biosample Type
cell line
Replication type
isogenic
Description
shRNA knockdown against SRSF1 in HepG2 cells followed by RNA-seq. (SRSF1-BGHLV12)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.4 [2-1]9.5 [1-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:SRSF1-BGHLV12
Date submitted
September 29, 2014
Date released
October 16, 2014
Gene Silencing Series
ENCSR308EYX 
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv1Search for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens SRSF1ENCBS148BFZENCGM038MUNENCLB146QCP
11Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens SRSF1ENCBS066SXTENCGM038MUNENCLB556DKZ