Experiment summary for ENCSR084SCN
doi:10.17989/ENCSR084SCN
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Control type
- non-specific target
- Biosample summary
- Homo sapiens K562 expressing RNAi
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Control shRNA against no target in K562 cells followed by RNA-seq. (non-target BGKLV13)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.4 [1-1]9.3 [2-1]
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR449DXG
- Platform
- Illumina HiSeq 2000
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:non-target_BGKLV13
- Date submitted
- October 29, 2014
- Date released
- November 20, 2014
- Gene Silencing Series
- ENCSR335TEE , ENCSR655TZO , ENCSR819WUX , ENCSR791UFD , ENCSR999PXW , ENCSR849MTM , ENCSR821NVF , ENCSR610SZF
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
---|---|---|---|---|---|
1 | 1 | Homo sapiens K562 cell line expressing RNAi | ENCBS418OCT | ENCGM266AWP | ENCLB136JSZ |
2 | 1 | Homo sapiens K562 cell line expressing RNAi | ENCBS252OWV | ENCGM266AWP | ENCLB972QAM |