Experiment summary for ENCSR081EST
doi:10.17989/ENCSR081EST
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Control type
- non-specific target
- Biosample summary
- Homo sapiens K562 expressing RNAi
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RNA-seq on K562 cells treated with a control shRNA against no target. (K-NT-26B)
- Nucleic acid type
- RNA
- RNA integrity number
- 10
- Depleted in
- rRNA
- Size range
- >200
- Extraction method
- Trizol
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR449DXG
- Platform
- Illumina HiSeq 2000
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:K-NT-26B
- Date submitted
- May 30, 2014
- Date released
- June 30, 2014
- Gene Silencing Series
- ENCSR972GKH , ENCSR624FPZ
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens K562 cell line expressing RNAi | ENCBS092VTJ | ENCGM756KEY | ENCLB005KIJ |
| 1 | 1 | Homo sapiens K562 cell line expressing RNAi | ENCBS184UCR | ENCGM756KEY | ENCLB385PHF |