Experiment summary for ENCSR071LAB
doi:10.17989/ENCSR071LAB
Summary
- Status
- released
- Assay
- ChIP-seq (Control ChIP-seq)
- Control type
- input library
- Biosample summary
- Caenorhabditis elegans strain OP701 (unc-119(tm4063); wgIs701 [sem-2::TY1::EGFP::3xFLAG + unc-119(+)]) whole organism mixed stage (embryo) (1 hour) using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans sem-2
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- This is the ChIP-seq control of transgenic worms expressing sem-2-eGFP fusion proteins.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:SEM-2_OP701_EE-INPUT
- Date submitted
- February 26, 2018
- Date released
- March 1, 2018
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain OP701 (unc-119(tm4063); wgIs701 [sem-2::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex mixed stage (embryo) (1 hour) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans sem-2 | ENCBS326CSK | ENCGM880AMQ | ENCLB472ZPS |
| 3 | 1 | Caenorhabditis elegans strain OP701 (unc-119(tm4063); wgIs701 [sem-2::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex mixed stage (embryo) (1 hour) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans sem-2 | ENCBS863LHQ | ENCGM880AMQ | ENCLB553QHS |