Experiment summary for ENCSR068SJG
doi:10.17989/ENCSR068SJG
Summary
- Status
- archived
- Assay
- single-cell RNA sequencing assay (scRNA-seq)
- Biosample summary
- Mus musculus strain B6CASTF1/J Purkinje cell male adult (14 months)
- Biosample Type
- primary cell
- Replication type
- unreplicated
- Description
- RNA-seq of a single Purkinje cell from a 14 month old male hybrid Castaneus X C57Bl6 mouse
- Nucleic acid type
- RNA
- Library starting quantity
- 1cells
- Size range
- >200
- Lysis method
- Clontech UltraLow for Illumina sequencing
- Size selection method
- SPRI beads
- Strand specificity
- unstranded
- Spike-ins datasets
- ENCSR013YHQ, ENCSR133ALU
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
ENCODE3 project
- Lab
- Barbara Wold, Caltech
- Award
- U54HG006998 (Richard Myers, HAIB)
- Project
- ENCODE
- External resources
- Date submitted
- June 25, 2015
- Date released
- October 13, 2015
- Submitter comment
- Estimates of technical measurement error from low input mass RNA-seq libraries.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Mus musculus strain B6CASTF1/J male adult (14 months) Purkinje primary cell | ENCBS530JMM | ENCLB322YDP |