Experiment summary for ENCSR042QTH
doi:10.17989/ENCSR042QTH
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Control type
- non-specific target
- Biosample summary
- Homo sapiens HepG2 expressing RNAi
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Control shRNA against no target in HepG2 cells followed by RNA-seq. (non-target-BGHLV23)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.7 [1-1]9.6 [2-1]
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR449DXG
- Platform
- Illumina HiSeq 2000
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:non-target-BGHLV23
- Date submitted
- August 13, 2015
- Date released
- August 18, 2015
- Gene Silencing Series
- ENCSR994ZMV , ENCSR560IJH , ENCSR139HZN , ENCSR721OFF , ENCSR030SUW , ENCSR439GMT , ENCSR885LXL , ENCSR736EJC , ENCSR281SSX , ENCSR239VHY , ENCSR561ONH , ENCSR979YNN , ENCSR674VQM , ENCSR200UMM , ENCSR955ACR , ENCSR826GBS
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
---|---|---|---|---|---|
1 | 1 | Homo sapiens HepG2 cell line expressing RNAi | ENCBS745UGC | ENCGM266AWP | ENCLB758GJZ |
2 | 1 | Homo sapiens HepG2 cell line expressing RNAi | ENCBS074PUV | ENCGM266AWP | ENCLB967MGK |