Experiment summary for ENCSR007XKL

doi:10.17989/ENCSR007XKL

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
NFX1
Biosample summary
Homo sapiens K562 expressing RNAi targeting H. sapiens NFX1
Biosample Type
cell line
Replication type
isogenic
Description
shRNA knockdown against NFX1 in K562 cells followed by RNA-seq. (NFX1 BGKLV13)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.5 [1-1]9.4 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:NFX1_BGKLV13
Date submitted
October 29, 2014
Date released
November 20, 2014
Gene Silencing Series
ENCSR440WRA 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line expressing RNAi targeting H. sapiens NFX1ENCBS143LJIENCGM196PFXENCLB180VMG
21Homo sapiens K562 cell line expressing RNAi targeting H. sapiens NFX1ENCBS654OPTENCGM196PFXENCLB120KJG