Experiment summary for ENCSR000EEO

Summary

Status
released
Assay
ChIP-seq
Target
SREBF1
Biosample summary
Homo sapiens HepG2 treated with 10 μM 22-hydroxycholesterol for 6 hours, 100 nM insulin for 6 hours
Biosample Type
cell line
Replication type
isogenic
Description
SREBF1 ChIP-seq on human HepG2 treated with insulin
Treatments
10 μM 22-hydroxycholesterol for 6 hour, 100 nM insulin (UniProtKB:P01308) for 6 hour
Nucleic acid type
DNA
Lysis method
see document
Extraction method
see document
Fragmentation method
see document
Size selection method
see document
Strand specificity
Non-strand-specific
Controls

Attribution

ENCODE2 project
Lab
Michael Snyder, Stanford
Award
U54HG004558 (Michael Snyder, Stanford)
Project
ENCODE
Date submitted
October 29, 2011
Date released
October 29, 2011
Tags
Search for all Experiment with internal tag ENCYCLOPEDIAv4

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleAntibodyLibrary
31HepG2 cell line treated with 10 μM 22-hydroxycholesterol for 6 hours, 100 nM insulin for 6 hoursENCBS849AAAENCAB000ALCENCLB209AHI
11HepG2 cell line treated with 10 μM 22-hydroxycholesterol for 6 hours, 100 nM insulin for 6 hoursENCBS849AAAENCAB000ALCENCLB209AJB
21HepG2 cell line treated with 10 μM 22-hydroxycholesterol for 6 hours, 100 nM insulin for 6 hoursENCBS849AAAENCAB000ALCENCLB209AJC