Experiment summary for ENCSR000AFD

  • Status: released

Summary

Assay
RNA-seq (total RNA-seq)
Biosample summary
Homo sapiens occipital lobe female fetal (20 weeks) and male fetal (22 weeks)
Biosample Type
tissue
Replication type
anisogenic
Description
The libraries contained in this experiment come from human fetal occipital lobe tissue from independent donors. They are stranded PE101 Illumina Hi-Seq RNA-Seq libraries from rRNA-depleted Total RNA > 200 nucleotides in size.
Nucleic acid type
RNA
Depleted in
rRNA
Size range
>200
Lysis method
see document
Extraction method
see document
Fragmentation method
see document
Size selection method
see document
Strand specificity
Strand-specific
Spike-ins datasets
ENCSR470JZL
Platform
HiSeq 2000

Attribution

ENCODE3 project
Lab
Thomas Gingeras, CSHL
Award
U54HG007004 (Thomas Gingeras, CSHL)
Project
ENCODE
External resources
Date released
2014-06-30
Tags
ENCYCLOPEDIAv4 tag

Anisogenic replicates

Anisogenic replicateTechnical replicateSummaryBiosampleLibrary
11occipital lobe tissue female fetal (20 weeks)ENCBS063RNAENCLB191ZZZ
21occipital lobe tissue male fetal (22 weeks)ENCBS064RNAENCLB192ZZZ

Documents

data sheet
Description: Ambion spike-in concentrations and subgroups for mix 1 and mix 2
Submitted by
Carrie Davis
Lab
Thomas Gingeras, CSHL
data sheet
Description: BioChain Human fetal occipital lobe B608332 data sheet
Submitted by
Carrie Davis
Lab
Thomas Gingeras, CSHL
data sheet
Description: BioChain Human fetal occipital lobe B608331 data sheet
Submitted by
Carrie Davis
Lab
Thomas Gingeras, CSHL
general protocol
Description: Specific protocol for library ENCLB192ZZZ
Submitted by
Carrie Davis
Lab
Thomas Gingeras, CSHL
general protocol
Description: Specific protocol for library ENCLB191ZZZ
Submitted by
Carrie Davis
Lab
Thomas Gingeras, CSHL
pipeline protocol
Description excerpt: Long RNA-seq pipeline: Includes overview, references, and spike-ins information about the pipeline
Description
Long RNA-seq pipeline: Includes overview, references, and spike-ins information about the pipeline
Submitted by
Kathrina Onate
Lab
J. Michael Cherry, Stanford