ENCBS963BLD / cell line

Summary

Status
released
Term name
MCF-7
Summary
Homo sapiens MCF-7 cell line expressing RNAi
Description
MCF-7 cells treated with a siRNA knockdown against a non-specific target control, used in RNA-seq experiments.

Attribution

ENCODE3 project
Lab
Peggy Farnham, USC
Award PI
Michael Snyder, Stanford
Submitted by
Heather Witt
Source
ATCC
Project
ENCODE
Aliases
peggy-farnham:B-MCF-7-siRNA-control-rep1

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM752AFWinterferencenon-specific target controlRNAi

Donor information

Status
released
Accession
ENCDO000AAE
Aliases
encode:donor of MCF-7, bradley-bernstein:Donor of MCF-7 cells
Species
Homo sapiens
Life stage
adult
Age
69 years
Sex
female
Health status
breast cancer (adenocarcinoma)
Ethnicity
Caucasian
External resources
References

Documents

transfection protocol
Description: Basic transfection protoco with Dharmafectl from Dharmacon
Submitted by
Heather Witt
Lab
Peggy Farnham, USC
other
Description: Basic siRNA suspension protocol from Dharmacon
Submitted by
Heather Witt
Lab
Peggy Farnham, USC