1. Biosamples
  2. cell line
  3. Homo sapiens
  4. SK-N-SH

ENCBS924BFP / cell line

Summary

Status
released
Term name
SK-N-SH
Summary
Homo sapiens SK-N-SH cell line genetically modified (insertion) using CRISPR targeting H. sapiens CREB5
Separated from biosample
ENCBS560KOF
Parent of biosamples
ENCBS860TSU, ENCBS532CTN

Attribution

ENCODE4 project
Lab
Michael Snyder, Stanford
Award PI
Michael Snyder, Stanford
Submitted by
Nathaniel Watson
Project
ENCODE
External resources
Aliases
michael-snyder:pB-11314, michael-snyder:pB-eGFP-CREB5 SK-N-SH

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM035OLJinsertiontaggingCRISPR

Donor information

Status
released
Accession
ENCDO000ABD
Aliases
encode:donor of SK-N-SH, bradley-bernstein:Donor of SK-N-SH cells, encode:donor of SH-SY5Y
Species
Homo sapiens
Life stage
child
Age
4 years
Sex
female
Health status
Metastatic neuroblastoma from bone marrow
External resources
References

Documents

growth protocol
Description: Cell growth protocol for SK-N-SH cells (version 1).
Attachment PDF Icon
ENCODE4_Snyder_CellGrowth_SK-N-SH_v1.pdf
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
transfection protocol
Description excerpt: SK-N-SH transfection protocol - Version 3 (Replicates split right after…
Attachment PDF Icon
ENCODE4_Snyder_CellTransfection_SK-N-SH_v3.pdf
Description
SK-N-SH transfection protocol - Version 3 (Replicates split right after transfection, 200 µg/ml G418, 1M cells per replicate)
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford