ENCBS914TKP / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP745 (wgIs745 [mxl-3::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex L4 larva (48 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting mxl-3
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to MXL-3.
Life stage
L4 larva
Age
48 hours
Synchronization timepoint
L1 larva starved after bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
Aliases
valerie-reinke:MXL-3_GFP_OP745_L4_Rep2

Genetic modifications

Accession
Category
Purpose
Method
Site
ENCGM057IPXinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO152RWU
Aliases
robert-waterston:OP745
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
wgIs745 [mxl-3::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)
Sex
mixed
Strain name
OP745

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale