ENCBS890YJR / in vitro differentiated cells

Summary

Status
released
Term name
erythroblast
Term ID
CL:0000765
Summary
Mus musculus strain MEL erythroblast in vitro differentiated cells originated from MEL-GATA-1-ER treated with 1 μM estradiol for 48 hours
Description
MEL-GATA-1-ER, This is a mouse suspension cell line derived from MEL cells by stable transfection with a GATA-1-ER fusion protein construct as described by Choe et al., 2003 (Cancer Res 63, 6363-6369, 2003). These cells can be terminally differentiated into mature erythroid cells with Β-estradiol treatment
Life stage
unknown
Age
Unknown
Originated from biosample
ENCBS212ENC

Attribution

ENCODE Mouse Project
Lab
John Stamatoyannopoulos, UW
Award PI
John Stamatoyannopoulos, UW
Submitted by
John Stamatoyannopoulos
Project
ENCODE
External resources
Aliases
john-stamatoyannopoulos:DS15415

Treatment details

Treatment
1 μM estradiol (CHEBI:23965) for 48 hour
Type
chemical

Strain information

Status
released
Accession
ENCDO073AAA
Aliases
encode:MEL-strain
Donor external identifiers
Species
Mus musculus
Sex
unknown
Health status
unknown
Strain background
DBA/2
Strain name
MEL

Documents

differentiation protocol
Description excerpt: 48 hour differentiation time point of mG/ER cells towards red blood cells…
Description
48 hour differentiation time point of mG/ER cells towards red blood cells (protocol C)
Submitted by
Unknown User
Lab
Unknown Lab
growth protocol
Description: Propagation of Mouse MEL-GATA-1-ER cells protocol
Submitted by
John Stamatoyannopoulos
Lab
John Stamatoyannopoulos, UW