ENCBS882KSU / cell line

Summary

Status
released
Term name
HEK293
Summary
Homo sapiens HEK293 cell line genetically modified (insertion) using site-specific recombination targeting ZNF572 originated from HEK293
Description
HEK293 cell line stably expressing N-terminal tagged eGFP-ZNF572 under the control of a CMV promoter.
Originated from biosample
ENCBS749QJL

Attribution

ENCODE3 project
Lab
Michael Snyder, Stanford
Award PI
Michael Snyder, Stanford
Submitted by
Tejaswini Mishra
Project
ENCODE
Aliases
michael-snyder:HEK_eGFP-ZNF572_FRT_ChIP_B1
Submitter comment
WARNING: The tagged TF could not be verified by AP-MS and may not reflect the annotated zinc finger target in the metadata.

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM699SGBinsertiontaggingsite-specific recombination
Integration site
random

Donor information

Status
released
Accession
ENCDO000ABF
Aliases
encode:donor of HEK293, encode:donor of HEK293T
Species
Homo sapiens
Life stage
embryonic
Age
Unknown
Sex
female
Health status
unknown
External resources

Documents

transfection protocol
Description: Stable cell line generation with Thermo Fisher's Flp-In system.
Attachment from submitter
Submitted by
Tejaswini Mishra
Lab
Michael Snyder, Stanford