ENCBS877BNV / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP383 (unc-119(tm4063) III; wgIs383) hermaphrodite L4 larva 52 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans oef-1
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to F49E8.2
Life stage
L4 larva
Age
Unknown
Synchronization timepoint
L1 larva starved after bleaching + 52 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:F49E8.2_GFP_OP383_L4_Rep3

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM637QPJinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO241VWT
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063) III; wgIs383.
Sex
hermaphrodite
Strain name
OP383
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale