ENCBS828BFU / cell line
Summary
- Status
- released
- Term name
- HepG2
- Term ID
- EFO:0001187
- Summary
- Homo sapiens HepG2 cell line genetically modified (insertion) using CRISPR targeting H. sapiens RAD21
- Description
- HepG2 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to RAD21.
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Nathaniel Watson
- Source
- Michael Snyder
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:HepG2_eGFP-RAD21_CRISPR_ChIP_B2
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM798LWU | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAC
- Aliases
- encode:donor of HepG2, bradley-bernstein:Donor of HepG2
- Species
- Homo sapiens
- Life stage
- child
- Age
- 15 years
- Sex
- male
- Health status
- hepatocellular carcinoma
- Ethnicity
- European
- External resources
- References
Documents
immunoblot
Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…
- Caption
- Immunoprecipitation was performed on nuclear extracts from biosample ENCBS390CJI (HepG2 eGFP-RAD21 replicate 1) cells using anti-eGFP antibody. The image shows western blot analysis of input (lane 1), immunoprecipitate (lane 2), and mock immunoprecipitate using IgG (lane 3). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD). The target molecular weight is 100.0 kD as indicated with an arrow. Lower size bands which may be due to potential degradation products are marked with asterisks.
- Submitted by
- Ingrid Youngworth
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442
- Documents