ENCBS727FRT / primary cell

Summary

Status
released
Term name
T-helper 2 cell
Term ID
CL:0000546
Summary
Homo sapiens male adult (35 years) T-helper 2 primary cell treated with 100 ng/mL Interleukin-4 for 36 hours, 5 μg/mL Interferon-gamma antibody for 36 hours
Starting amount
500000cells
Separated from biosample
ENCBS069KZY

Attribution

ENCODE4 project
Lab
Yijun Ruan, JAX
Award PI
Yijun Ruan, JAX
Submitted by
Bonita Lam
Source
AllCells
Project
ENCODE
External resources
Aliases
barbara-wold:CD4-Naive-Th2-Resting_LP-18_2

Treatment details

Treatment
100 ng/mL Interleukin-4 (UniProtKB:P05112) for 36 hours
Type
protein
Purpose
activation
Treatment
5 μg/mL Interferon-gamma antibody for 36 hours
Type
antibody
Purpose
activation

Donor information

Status
released
Accession
ENCDO374BBL
Aliases
yijun-ruan:donor_LP-18
Donor external identifiers
  • AllCells:10477
Species
Homo sapiens
Life stage
adult
Age
35 years
Sex
male
External resources
Submitter comment
AllCells:10477

Documents

cell isolation protocol
Description excerpt: Protocol describing the purification, activation, and differentiation of…
Description
Protocol describing the purification, activation, and differentiation of various immune cells. This document is an updated version of /documents/f1b60b4e-b5d2-4b8d-af0b-06b767fa07c3/.
Submitted by
Jennifer Jou
Lab
Charles Lee