ENCBS723SCX / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain CS152 (sma-3(wk30);qcIs[gfp::sma-3+rol-6]) hermaphrodite L2 larva 24 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using microinjection targeting C. elegans sma-3
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to SMA-3
Life stage
L2 larva
Age
Unknown
Synchronization timepoint
L1 larva starved after bleaching + 24 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:SMA-3_GFP_CS152_L2_INPUT

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM504MNXinsertiontaggingmicroinjection
Coordinates
ce10 chrIII:6859735-6867560

Strain information

Status
released
Accession
ENCDO022KND
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
sma-3(wk30);qcIs[gfp::sma-3+rol-6]
Sex
hermaphrodite
Strain name
CS152
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale