ENCBS715FVA / primary cell
Summary
- Status
- released
- Term name
- activated naive CD8-positive, alpha-beta T cell
- Term ID
- NTR:0000502
- Summary
- Homo sapiens male adult (30 years) activated naive CD8-positive, alpha-beta T primary cell treated with anti-CD3 and anti-CD28 coated beads for 7 days, 10 ng/mL Interleukin-2 for 5 days
- Starting amount
- 2000000cells
- Separated from biosample
- ENCBS448XFR
Attribution
ENCODE4 project
- Lab
- Yijun Ruan, JAX
- Award PI
- Yijun Ruan, JAX
- Submitted by
- Bonita Lam
- Source
- AllCells
- Project
- ENCODE
- External resources
- Aliases
- bradley-bernstein:BioSam 4340, bradley-bernstein:CD8-Naive-Day7-Activation
Treatment details
- Treatment
- anti-CD3 and anti-CD28 coated beads for 7 days
- Type
- antibody
- Purpose
- activation
- Treatment
- 10 ng/mL Interleukin-2 (UniProtKB:P60568) for 5 days
- Type
- protein
- Purpose
- stimulation
Donor information
- Status
- released
- Accession
- ENCDO166AGR
- Aliases
- yijun-ruan:donor_LP-14
- Donor external identifiers
- AllCells:11748
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 30 years
- Sex
- male
- Ethnicity
- European
- External resources
- Submitter comment
- AllCells:11748
Documents
cell isolation protocol
Description excerpt: Protocol describing the purification, activation, and differentiation of…
- Description
- Protocol describing the purification, activation, and differentiation of various immune cells. This document is an updated version of /documents/f1b60b4e-b5d2-4b8d-af0b-06b767fa07c3/.
- Submitted by
- Jennifer Jou
- Lab
- Charles Lee
- Grant
- UM1HG009409