ENCBS693BEC / primary cell
Summary
- Status
- released
- Term name
- activated T-cell
- Term ID
- NTR:0000537
- Summary
- Homo sapiens male adult (38 years) activated T-cell primary cell treated with anti-CD3 and anti-CD28 coated beads for 24 hours, 50 U/mL Interleukin-2 for 24 hours
- Description
- Activated with 1:1 ratio of CD3, CD28 beads for 24 hours by Stam lab at Altius.
- Separated from biosample
- ENCBS708JOR
Attribution
ENCODE4 project
- Lab
- Bradley Bernstein, Broad
- Award PI
- Bradley Bernstein, Broad
- Submitted by
- Noam Shoresh
- Source
- John Stamatoyannopoulos
- Project
- ENCODE
- External resources
- Date obtained
- 2019-10-25
- Aliases
- bradley-bernstein:BioSam 4021
Treatment details
- Treatment
- anti-CD3 and anti-CD28 coated beads for 24 hours
- Type
- antibody
- Purpose
- activation
- Treatment
- 50 U/mL Interleukin-2 (UniProtKB:P60568) for 24 hours
- Type
- protein
- Purpose
- activation
Donor information
- Status
- released
- Accession
- ENCDO923WRX
- Aliases
- bradley-bernstein:Donor 1281
- Donor external identifiers
- AllCells:15824
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 38 years
- Sex
- male
- External resources
Documents
cell isolation protocol
Description excerpt: Isolation of Immune cells by Stemcell Technologies Magnetic Beads kits and…
- Description
- Isolation of Immune cells by Stemcell Technologies Magnetic Beads kits and stimulations (Stamatoyannopoulos lab). This protocol is an updated version of : https://www.encodeproject.org/documents/b94d1a22-1ef2-4820-9b71-cdcfba41127c/
- Submitted by
- Jennifer Jou
- Lab
- John Stamatoyannopoulos, UW
- Grant
- UM1HG009444