ENCBS674LEN / primary cell
Summary
- Status
- released
- Term name
- activated T-helper 17 cell
- Term ID
- NTR:0000635
- Summary
- Mus musculus strain C57BL/6NJ activated T-helper 17 primary cell genetically modified (episome) using transient transfection treated with 0.25 μg/mL anti-CD3e for 3 days, 1 μg/mL anti-CD28 for 3 days, 2 μg/mL anti-Interleukin-4 for 3 days, 2 μg/mL anti-Interferon-gamma for 3 days, 10 ng/mL Interleukin-6 for 3 days, 0.3 ng/mL Transforming growth factor beta-1 proprotein for 3 days
- Life stage
- unknown
- Age
- Unknown
Attribution
ENCODE4 project
- Lab
- Tim Reddy, Duke
- Award PI
- Tim Reddy, Duke
- Submitted by
- Revathy Venukuttan
- Source
- Maria Ciofani
- Project
- ENCODE
- Aliases
- tim-reddy:Th17-atac-starrseq-biosample-rep1
Treatment details
- Treatment
- 0.25 μg/mL anti-CD3e for 3 days
- Type
- antibody
- Purpose
- activation
- Treatment
- 1 μg/mL anti-CD28 for 3 days
- Type
- antibody
- Purpose
- activation
- Treatment
- 2 μg/mL anti-Interleukin-4 for 3 days
- Type
- antibody
- Purpose
- activation
- Treatment
- 2 μg/mL anti-Interferon-gamma for 3 days
- Type
- antibody
- Purpose
- activation
- Treatment
- 10 ng/mL Interleukin-6 (UniProtKB:P08505) for 3 days
- Type
- protein
- Purpose
- activation
- Treatment
- 0.3 ng/mL Transforming growth factor beta-1 proprotein (UniProtKB:P01137) for 3 days
- Type
- protein
- Purpose
- activation
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM741TNP | episome | characterization | transient transfection |
Strain information
- Status
- released
- Accession
- ENCDO236IKC
- Aliases
- encode:C57BL_6NJ
- Donor external identifiers
- Species
- Mus musculus
- Sex
- unknown
- Strain background
- C57BL/6
- Strain name
- C57BL/6NJ