ENCBS658TJZ / cell line

Summary

Status
released
Term name
GM23338
Summary
Homo sapiens GM23338 cell line genetically modified (insertion) using CRISPR targeting H. sapiens ZNF114
Separated from biosample
ENCBS864AKW
Parent of biosamples
ENCBS274RDI, ENCBS587JWF

Attribution

ENCODE4 project
Lab
Michael Snyder, Stanford
Award PI
Michael Snyder, Stanford
Submitted by
Nathaniel Watson
Project
ENCODE
External resources
Aliases
michael-snyder:pB-11166, michael-snyder:pB-eGFP-ZNF114 PGP1

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM231ANFinsertiontaggingCRISPR

Donor information

Status
released
Accession
ENCDO336AAA
Aliases
bradley-bernstein:Donor hu43860C, encode:donor of hu43860C
Donor external identifiers
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
male
Ethnicity
European
External resources

Documents

growth protocol
Description: Cell growth protocol for PGP1 cells (version 1).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
transfection protocol
Description excerpt: PGP1 transfection protocol - Version 1 (Replicates split right after…
Description
PGP1 transfection protocol - Version 1 (Replicates split right after transfection, 30 µg/ml G418, mTeSR medium)
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford