ENCBS585DSE / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP543 hermaphrodite young adult 64 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans sdz-38
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to SDZ-38
Life stage
young adult
Age
Unknown
Synchronization timepoint
L1 larva starved after bleaching + 64.0 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:SDZ-38_GFP_OP543_YA_INPUT

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM295MGQinsertiontaggingbombardment
Coordinates
ce11 chrII:9964292-10003531
Integration site
random

Strain information

Status
released
Accession
ENCDO462XER
Aliases
robert-waterston:OP543
Species
Caenorhabditis elegans
Sex
hermaphrodite
Strain name
OP543
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale