1. Biosamples
  2. whole organisms
  3. Caenorhabditis elegans
  4. whole organism

ENCBS574JIA / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP433 (unc-119(tm4063) III; wgIs433) hermaphrodite L4 larva whole organism genetically modified (insertion) using bombardment targeting C. elegans hlh-30
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to HLH-30
Life stage
L4 larva
Age
Unknown

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:HLH-30_GFP_OP433_L4_Rep1

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM986POWinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO958UOX
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063) III; wgIs433.
Sex
hermaphrodite
Strain name
OP433
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Attachment from submitters
OP433_HLH-30_L4.jpg
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale
general protocol
Description: Protocol used by the Reinke lab for ChIP-seq
Attachment PDF Icon
Reinke_Lab_ModENCODE_ChIP_protocol_(revised 5-24-13).pdf
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale