ENCBS570HIY / whole organisms
Summary
- Status
- released
- Term name
- whole organism
- Term ID
- UBERON:0000468
- Summary
- Drosophila melanogaster strain Notch-GFP wandering third instar larva whole organism stably expressing C-terminal eGFP-tagged N
- Life stage
- wandering third instar larva
- Age
- Unknown
Attribution
modENCODE Project
- Lab
- Kevin White, UChicago
- Award PI
- Kevin White, UChicago
- Submitted by
- Kevin White
- Source
- Kevin White
- Project
- modENCODE
- Aliases
- kevin-white:B2-modencode_5011
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM864REG | insertion | tagging | stable transfection |
|
Strain information
- Status
- released
- Accession
- ENCDO995XNU
- Aliases
- modencode:Notch-GFP
- Species
- Drosophila melanogaster
- Genotype
- PBac{y[+]-attP-3B}VK00033
- Sex
- unknown
- Strain name
- Notch-GFP
Documents
immunofluorescence
Caption excerpt: Strain name: Notch-GFP
Genotype: PBac{y[+]-attP-3B}VK00033
Transgene: Notch…
- Caption
- Strain name: Notch-GFP Genotype: PBac{y[+]-attP-3B}VK00033 Transgene: Notch genomic coding region, stop codon replaced with GFP Short Description: P[acman] BAC number CH321-89E02 has been chosen to target the gene Notch. The LAP-TAG containing the GFP has been recombineered in 3' of the gene in frame. The engineered vector has then been inserted into the specific docking site with the help of Att sites. Recombinant individuals were screened based on mini-white presence. Tag Type: LAP-TAG_EGFP Vector Used: P[acman] Genomic Coordinates: X from 2986257 to 3075513 attP Coordinates: 3L:6435776..6435776 Made_by: Alec Victorsen, Rebecca Spokony
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U01HG004264