ENCBS554UZW / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) treated with 1 μg/mL doxycycline for 48 hours
- Culture start date
- 2020-10-09
- Culture harvest date
- 2020-12-09
- Passage number
- 20
Attribution
ENCODE4 project
- Lab
- Pardis Sabeti, Broad
- Award PI
- Pardis Sabeti, Broad
- Submitted by
- Ava Mackay-Smith
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- pardis-sabeti:K562_chr6_134965279_135054691_HEME-HBS1L_R1
Treatment details
- Treatment
- 1 μg/mL doxycycline (CHEBI:50845) for 48 hours
- Type
- chemical
- Purpose
- activation
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM097HOE | insertion | expression | transduction | ||
| ENCGM470LXQ | CRISPRi | characterization | CRISPR |
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References