ENCBS507ODV / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain YL487 hermaphrodite young adult 60 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans snpc-4
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to SNPC-4
Life stage
young adult
Age
Unknown
Synchronization timepoint
L1 larva starved after bleaching + 60 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:SNPC-4_GFP_YL487_yAd_Rep2

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM508IMVinsertiontaggingbombardment
Coordinates
ce11 chrI:8943797-8976824
Integration site
random

Strain information

Status
released
Accession
ENCDO079LSX
Aliases
valerie-reinke:YL487
Species
Caenorhabditis elegans
Sex
hermaphrodite
Strain name
YL487
External resources

Documents

general protocol
Description: Protocol used by the Reinke lab for ChIP-seq
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale
immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale