ENCBS491PRG / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP594 (unc-119(tm4063) III; wgIs594) mixed sex young adult 63 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-fkh-3
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to FKH-3
Life stage
young adult
Age
Unknown
Synchronization timepoint
L1 larva starved after bleaching + 63.0 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
Aliases
valerie-reinke:FKH-3_GFP_OP594_yAd_Rep1

Genetic modifications

AccessionCategoryPurposeMethodSite
ENCGM731KOLinsertiontaggingbombardment
Coordinates
ce11 chrX:13403136-13436729
Integration site
random

Strain information

Status
released
Accession
ENCDO989FSA
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063) III; wgIs594
Sex
mixed
Strain name
OP594

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale
general protocol
Description: Protocol used by the Reinke lab for ChIP-seq
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale